Plant J|山东农大郝玉金团队揭示MdABI5与其互作伙伴协同调节脱落酸介导的苹果叶片衰老

脱落酸（ABA）诱导叶绿素降解和叶片衰老。然而，其分子机制尚不清楚，尤其是在木本植物中。本研究发现MdABI5在ABA诱导的苹果叶片衰老过程中起着重要的调控作用。通过酵母筛选，发现MdBBX22、MdWRKY40和MdbZIP44三种转录因子在体内外与MdABI5直接相互作用。生理生化分析表明，MdBBX22在两个途径上延缓了叶片衰老。首先，MdBBX22与MdABI5相互作用，抑制MdABI5在叶绿素分解代谢基因MdNYE1和MdNYC1上的转录活性，从而负调控叶绿素降解和叶片衰老。其次，MdBBX22与MdHY5相互作用，干扰MdHY5在MdABI5上的转录激活，从而抑制MdABI5的表达，从而延缓叶片衰老。MdWRKY40和MdbZIP44是叶片衰老的阳性调节因子。它们通过相同的调控途径加速MdABI5促进叶片衰老，即与MdABI5相互作用，增强MdABI5在mdny1和MdNYC1上的转录活性。综上所述，我们的研究结果表明，MdABI5与其正或负调控互作因子共同调控ABA介导的苹果叶片衰老，而MdABI5是其中的核心调控因子。这些拮抗调控途径保证了植物对外界胁迫反应的灵活性和高效性。本研究结果为进一步研究叶片衰老的调控机制提供了思路。Abscisic acid (ABA) induces chlorophyll degradation and leaf senescence. However, the molecular mechanism remains poorly understood, especially in woody plants. In this study, we found that MdABI5 plays an essential role in the regulation of ABA‐triggered leaf senescence in apple. Through yeast screening, three transcription factors, MdBBX22, MdWRKY40, and MdbZIP44, were found to interact directly with MdABI5 in vitro and in vivo. Physiological and biochemical assays showed that MdBBX22 delayed leaf senescence in two pathways. First, MdBBX22 interacted with MdABI5 to inhibit the transcriptional activity of MdABI5 on chlorophyll catabolic genes MdNYE1 and MdNYC1, thus negatively regulating chlorophyll degradation and leaf senescence. Second, MdBBX22 interacted with MdHY5 to interfere with the transcriptional activation of MdHY5 on MdABI5, thereby inhibiting the expression of MdABI5, which also contributed to the delay of leaf senescence. MdWRKY40 and MdbZIP44 were identified as positive regulators of leaf senescence. They accelerated MdABI5‐promoted leaf senescence through the same regulatory pathways, that is, interacting with MdABI5 to enhance the transcriptional activity of MdABI5 on MdNYE1 and MdNYC1. Taken together, our results suggest that MdABI5 works with its positive or negative interaction partners to regulate ABA‐mediated leaf senescence in apple, in which it acts as a core regulator. The antagonistic regulation pathways ensure that plants respond to external stresses flexibly and efficiently. Our results provide a concept for further study on the regulation mechanisms of leaf senescence.An, J.‐P., Zhang, X.‐W., Liu, Y.‐J., Zhang, J.‐C., Wang, X.‐F., You, C.‐X. and Hao, Y.‐J. (2020), MdABI5 works with its interaction partners to regulate abscisic acid‐mediated leaf senescence in apple. The Plant Journal. Accepted Author Manuscript. https://doi.org/10.1111/tpj.15132

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