＇长江下游麦区新育成品种(系) 3种主要病害的抗性鉴定及抗病基因/QTL的分子检测
2021,Vol. 47(12): 2335-2347
作物遗传育种·种质资源·分子遗传学
长江下游麦区新育成品种(系) 3种主要病害的抗性鉴定及抗病基因/QTL的分子检测
吕国锋，别同德，王慧，赵仁慧，范金平，张伯桥，吴素兰，王玲，汪尊杰，高德荣
江苏里下河地区农业科学研究所 / 农业农村部长江中下游小麦生物学与遗传育种重点实验室, 江苏扬州 225007
Evaluation and molecular detection of three major diseases resistance of new bred wheat varieties (lines) from the lower reaches of the Yangtze River
LYU Guo-Feng,BIE Tong-De,WANG Hui,ZHAO Ren-Hui,FAN Jin-Ping,ZHANG Bo-Qiao,WU Su-Lan,WANG Ling,WANG Zun-Jie,GAO De-Rong
Lixiahe Institute of Agricultural Sciences / Key Laboratory of Wheat Biology and Genetic Improvement for Lower & Middle Yangtze Valley, Ministry of Agriculture and Rural Affairs, Yangzhou 225007, Jiangsu, China
摘要： 小麦赤霉病、白粉病和黄花叶病是长江下游麦区小麦生产的主要病害。本研究对长江下游麦区新育成49个品种(系)的上述3 (种病害进行抗性鉴定，同时利用与抗赤霉病主效QTL Fhb1和QFhs.crc-2D、抗白粉病基因Pm21以及抗黄花叶病主效QTL QYm.nau-5A.1和QYm.nau-2D连锁的分子标记检测试验品种3种病害抗病基因/QTL组成。结果显示，49.0%的品种赤霉病抗性达中抗以上，32.6%的品种对白粉病免疫或抗，44.9%的品种抗黄花叶病。30.6%和73.6%的试验品种分别含有抗赤霉病主效QTL Fhb1和QFhs.crc-2D，宁麦9号和扬麦158及其衍生品种分别是Fhb1和QFhs.crc-2D的主要载体品种；28.6%的品种含抗白粉病基因Pm21，镇麦9号和扬麦18及其衍生品种为Pm21的主要载体品种；分子检测含抗黄花叶病主效QTL QYm.nau-5A.1和QYm.nau-2D的品种比例均为24.5%，宁麦9号和苏麦6号及其衍生品种分别是QYm.nau-5A.1和QYm.nau-2D的主要载体品种。宁麦9号和扬麦158衍生品种在小麦抗赤霉病和黄花叶病基因/QTL的组成存在分化。结果为长江下游麦区小麦抗病分子育种提供了重要参考。
关键词： 小麦, 品种, 抗病QTL/基因, 分子鉴定
Abstract: Fusarium head blight (FHB), powdery mildew (PM), and wheat yellow mosaic virus (WYMV) are three major diseases of wheat in the lower reaches of Yangtze River. The resistance to FHB, PM, and WYMV of 49 new bred wheat varieties (advanced lines) from the lower reaches of the Yangtze River were evaluated, and the molecular markers linked to FHB resistance QTL Fhb1 and QFhs.crc-2D, PM gene Pm21 and WYMV resistance QTL QYm.nau-5A.1 and QYm.nau-2D wereused to detect the correspond resistance genes / QTLs. 49.0% of the varieties were above moderate resistance to FHB, 32.6% varieties were immune or resistant to PM, and 44.9% varieties were resistant to WYMV. 30.6% and 73.6% of the tested varieties contained Fhb1 and QFhs.crc-2D, respectively. Ningmai 9 and Yangmai 158 as well as their derived varieties were the main carriers of Fhb1 and QFhs.crc-2D, respectively. Pm21, a powdery mildew resistant gene, was detected in 28.6% of the tested varieties, Zhenmai 9 and Yangmai 18 were two major donors of Pm21. QYm.nau-5A.1 and QYm.nau-2D, two MYMV resistant QTL, were detected in 24.5% of the tested varieties. Ningmai 9 and Zhenmai 9 were two major QTL donors. The composition of resistance genes/QTLs to FHB and WYMV in Yangmai 158 and their derived varieties diverged from Ningmai 9 and its derived varieties. These results provide the important information for molecular breeding for wheat disease resistance in lower reaches of Yangtze River.
Key words: wheat, varieties, disease resistant genes/QTLs, molecular detection
收稿日期: 2020-12-15
基金资助: 本研究由国家重点研发计划项目(2017YFD0100800)和江苏省重点研发计划项目(BE2018350)资助。
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