反复接触七氟醚会损害老年雄性大鼠的学习能力和记忆
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Repeated exposure to sevoflurane impairs the learning and memory of older male rats
背景与目的:危重病患者常需要反复手术干预，因此探讨反复接触麻醉药物对学习和记忆的影响就显得非常重要。七氟醚是一种广泛使用的吸入麻醉剂，对神经系统几乎没有副作用，并能使意识迅速恢复。但七氟醚暴露的长期影响和重复七氟醚暴露后对认知功能的影响却少有文献报道，这与现有的研究相矛盾。
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方法：在本研究中，Morris水迷宫试验被用来研究单次（4小时）或重复（每天2小时连续5天）暴露于1.5％或2.5％七氟醚后对老年（16-18月龄）雄性大鼠学习能力和记忆的长期影响。测试是从最后一次暴露后1天到4周内进行。在海马中，通过对脑源性神经营养因子（BDNF），NF-κBmRNA和细胞凋亡率进行检验，以确定在单次或重复接触七氟醚后对认知功能和记忆的影响是否与细胞生化的改变有关。
结果：反复接触2.5％七氟醚降低了老年雄性大鼠海马的BDNF蛋白水平，提高了海马的NF-κBmRNA水平，增加了锥体细胞凋亡率。单次暴露于2.5％七氟醚以及反复接触1.5％或2.5％七氟醚显着损害了老年雄性大鼠的学习能力和记忆。
结论：反复接触七氟醚会损害老年雄性大鼠的学习能力和记忆，这种损伤伴随着海马内与认知功能相关的生化改变。
Guo S1, Liu L2, Wang C2, Jiang Q2, Dong Y2, Tian Y3. Repeated exposure to sevoflurane impairs the learning and memory of older male rats.
Life Sci. Nov 16，2017. pii: S0024-3205(17)30602-1. doi: 10.1016/j.lfs.2017.11.025. [Epub ahead of print]
Aims: Critically ill old patients sometimes require repeated surgical interventions, and thus it is important to determine the influence of repeated exposure to anesthetics on learning and memory. Sevoflurane, a widely used inhalation anesthetic, has few neurological adverse effects and offers a rapid return to consciousness. But the long-term influence of sevoflurane exposure and the effect of repeated sevoflurane exposure on cognition have rarely been reported, and available studies are contradictory.
Materials and methods: In the present study, the Morris water maze test was employed to investigate the longterm influence of single (4 h) or repeated (2 h daily for 5 consecutive days) exposure to 1.5% or 2.5% sevoflurane on the learning ability and memory of old (16–18 months old) male rats. Testing was performed from 1 day to 4 weeks after the last exposure. In the hippocampus, brain derived neurotrophic factor (BDNF), NF-κB mRNA, and apoptosis rate were also examined to determine whether cellular biochemical changes related to cognition and memory occurred after single or repeated exposure to sevoflurane.
Key findings: Repeated exposure to 2.5% sevoflurane decreased hippocampal levels of BDNF protein, enhanced hippocampal levels of NF-κB mRNA, and increased the apoptosis rate of pyramidal cells. Single exposure to 2.5% sevoflurane, and repeated exposure to either 1.5% or 2.5% sevoflurane significantly compromised learning and memory of old male rats.
Significance: Repeated exposure to sevoflurane impaired the learning and memory of old male rats, an impairment that was accompanied by cognition-related biochemical changes in the hippocampus.
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