双光子成像和近红外二区荧光共聚焦成像 二价锰离子磁共振成像MRI/纳米生物探针MRI显影剂

双光子成像和近红外二区荧光共聚焦成像 二价锰离子磁共振成像MRI/纳米生物探针MRI显影剂

我们使用 PTD 纳米颗粒实现了透过老鼠头骨脑血管的三维高分辨(分辨率 25.4 微米),高信号/背景比例( 22.3 dB)成像. 其成像深度高达 1001 微米. 该脑血管光声成像效果比较近报道的双光子成像和近红外二区荧光共聚焦成像的效果好.

▲Figure 2. PA imaging of subcutaneous HepG2 tumor-bearing mouse ear with a colorbar 0.06-1. (a) Photo of mouse ear bearing subcutaneous tumor for PA imaging. Representative xy projected tumor bearing mouse ear image (7.00 × 7.00 mm, x × y) before (b) and after (c) PTD NP administration. (d) Depth-encoded maximum amplitude projection image corresponding to Figure c (The PA signal color changes correspond to different depths according to the color chart for depth information on the right side). (e) and (f) 3D reconstruction of tumor-bearing mouse ear vasculature images from different view side (7.00 × 7.00 × 0.76 mm, x × y × z) and the tumor margin was labelled with white-dashed circle. (g) Layer-by-layer PA images (7.00 × 7.00 mm, x × y) of subcutaneous tumor-bearing mouse ear with white-dashed circle for labelling tumor margin in each layer. (h) and (i) The PA intensity profile (black curve) along the green line in the zoomed area (insets, Figures h and i) which represents the area labelled with green-dashed circle at depths of 370 and 755 µm, respectively. The Gaussian fits to the profiles are presented using red curves. Gaussian-fitted full width at half maximum (FWHM) of the vessel along the green line is presented at different depth.

▲Figure 3. In vivo ORPAMI of whole-cortex brain through intact skull after administration of PTD NPs through tail-vain (colorbar: 0.06-1). (a) Layer-by-layer PA images (8 × 6 mm, x × y) of mouse brain. The deepest area reached 1001 µm. (b) Photo of mouse for imaging. (c) Representative xz projected brain vasculature image (8 × 1 mm, x × z). (e) Representative xy projected brain vasculature image (8 × 6 mm, x × y). (f) 3D reconstruction of brain vasculature (8 × 6 × 1 mm, x × y × z). (d) and (g) The PA intensity profiles along the green line in the zoomed area (inset, Figure d and g) which represents the area labelled with green-dashed circle (Figure a) at the depths of 77 and 1001 µm, respectively. The Gaussian fits to the profile are shown in red curve. Gaussian-fitted full width at half maximum (FWHM) of the vessel along the green line is presented at different depth.

总结与展望

实现了体外造影剂辅助近红外二区光声显微成像. 微流控技术制备共轭高分子, 可以实现尺寸可控, 形貌均一. 同时,共轭高分子生物相容性好,吸光系数大,光声稳定性好,是很好的活体成像的光声造影剂. 我们证明二区共轭高分子辅助光声显微成像可以准确勾画肿瘤边界, 解析肿瘤内部和周围正常组织血管结构, 准确成像脑补三维复杂血管脉络. 因此, 共轭高分子纳米颗粒是很有潜力的活体成像造影剂, 用来理解生理和病理过程。

超声&MR成像对比剂（BDView-US/MRI）

UltrasoundMRimagingcontrastagent（超声影像；可同时递送磁共振T1影像分子）；粒径控制1000nm

超声荧光成像气泡（BDView-US/Tracer）

Ultrasoundfluorescenceimagingnanobubble（超声影像；可同时递送活体荧光造影剂）；粒径控制1000nm

超声成像阳离子微泡（BDView-US/Trans+）

Ultrasoundimagingcationicmicrobubbles（超声影像对比功能）；粒径控制1000nm

超声气泡对比剂（BDView-USPrime）

Ultrasoundcontrastbubble（超声影像功能）；粒径控制1000nm

超声释放核酸纳泡（BDView-US/Trans+）

Ultrasoundreleasingnucleicacidnanobubbles（超声影像；可超声响应释放药物分子）；粒径控制1000nm

超声释放紫杉醇微泡（BDView-US/Drug）

Ultrasoundreleaseofpaclitaxelmicrobubbles（超声影像；可超声响应释放紫杉醇分子）；粒径控制1000nm

释药型超声造影微泡（BDView-US/Drug）

Drugreleaseultrasoundmicrobubble（超声影像；可同时递送药物分子）；粒径控制1000nm

超声微纳气泡&磁共振双模影像对比剂（BDView-US/MRI）

Ultrasoundmicrobubbles&MRIdualmodeimagingcontrastagent（超声影像；可同时磁共振T1影像剂）；粒径控制1000nm

活体荧光&超声对比双模影像剂（BDView-US/Tracer）

Dualmodeimagingagentoffluorescenceultrasoundcontrast（超声影像；可同时递送活体荧光染料）；粒径控制1000nm

运载核酸型超声对比剂（BDView-US/Trans+）

BDView-us/trans+（超声影像；可同时递送核酸载体）；粒径控制1000nm

紫杉醇&超声微泡对比剂（BDView-US/Drug）

Paclitaxel&ultrasoundmicrobubblecontrastagent（超声影像；可同时递送药物紫杉醇）；粒径控制1000nm

超声纳米造影剂（BDView-USPrime）

Ultrasoundnanobubblecontrastagent（超声影像功能）；粒径控制1000nm

阿霉素&全氟烷超声微泡（BDView-US/Drug）

Adriamycinperfluoroalkaneultrasoundmicrobubbles（超声影像；可同时递送化学药物阿霉素）；粒径控制1000nm

全氟烷类超声对比微泡（BDView-USPrime）

Perfluoroalkaneultrasoundcontrastmicrobubbles（超声影像）；粒径控制1000nm

全氟烷-顺铂超声囊泡

PerfluoraneandCisplatinloadedultrasound-sensitiveNanobulble

全氟烷超声释药囊泡

UltrasoundResponsiveNanobulbleEncasultedPerfluorane

液态氟碳纳米粒造影剂

LiquidFluorocarbonContrastAgentLoadedNanocomplex

全氟丙烷微泡

PerfluoropropaneMicrobubbles

SPECT影像

影像双模态

磁共振T1/T2复合造影囊泡

T1/T2CompoundContrastPolymersome

超声-磁共振复合影像囊泡

ComplexedNanobulbleforUltrasoundandMRIImage

T1造影剂

Gd-DTPA

Gadolinium-LabeledAlbumin

Gadolinium-labeledIgG

Gadolinium-labeledProteinA

Gadolinium-labeledPolylysine

Gadolinium-labeledBSA

Gd-BSA-FITC

Gd-BSA-RhodamineB

Gd-DOTA

Gd-DO3A-Butylamine

DMPE-DTPA-Gd

DTPA-BSA-Gd

DPPE-DTPA-Gd

DSPE-DTPA-Gd

DMPE-DTPA-Gd-DMPE

DPPE-DTPA-Gd-DPPE

DSPE-DTPA-Gd-DSPE

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