整体解决方案：应对新冠突变株全方位研究的新冠突变体试剂库
|
中和抗体货号 |
WT RBD蛋白 (Cat:40592-V08B) |
N501Y突变蛋白 (Cat:40592-V08H82 ) |
K417N,E484K, N501Y突变蛋白 (Cat:40592-V08H85) |
|
40150-D001 |
245.5 |
466.9 |
404.5 |
|
40150-D002 |
253.4 |
769.2 |
484.6 |
|
40591-MM43 |
146.4 |
274.9 |
228.2 |
|
40592-MM57 |
475.4 |
934.2 |
- |
|
40592-R001 |
66.5 |
115.3 |
- |
|
40592-R118 |
20.4 |
97.8 |
- |
|
40592-R117 |
21.8 |
93.5 |
- |
注：WT: Wild Type；以上表格中数值为 IC50(ng/mL)
|
蛋白货号 |
突变位点 |
lineage |
|
40588-V07E1 |
N(R203K,G204R) |
高频突变，出现在B.1.1.7、B.1.351、P.1等 |
|
40588-V07E2 |
N(P13L) |
高频突变，出现在B.1.1.7、B.1.351、A.2.2等 |
|
40588-V07E3 |
N(S194L) |
高频突变，出现在B.1.1.7、B.1.237等 |
|
40588-V07E4 |
N(I292T) |
高频突变，出现在B.1.1.7等 |
|
40588-V07E8 |
N(D3L,S235F) |
B.1.1.7的特征突变 |
· 数据来源于GISAID
· GISAID促进快速共享导致COVID-19的所有冠状病毒的数据
· NP(R203K,G204R)是NP蛋白的上出现的高频突变，参考文献来源: https://doi.org/10.3389/fmicb.2020.01800
· NP(P13L), NP(S194L), NP(I292T)是NP蛋白的上出现的高频突变，参考文献来源于: https://doi.org/10.1002/jmv.26626
· 毒株信息：UK variant (lineage B.1.1.7), South Africa variant (lineage B.1.351), Brazil variant (lineage P.1)
新冠研究相关产品列表：
新冠蛋白产品
|
英国突变株 B.1.1.7 重要突变位点研究 |
||
|
货号 |
抗原 |
突变位点 |
|
40592-V02H1 |
RBD |
N501Y |
|
40592-V08H82 |
RBD |
N501Y |
|
40591-V08H7 |
S1 |
△H69/△V70，N501Y，D614G |
|
40591-V08H12 |
S1 |
H69del, V70del, Y1445del, N501Y, A570D, D614G, P681H |
|
40588-V07E7 |
NP |
D3L, R203K, G204R, S235F |
|
40588-V07E8 |
NP |
D3L, S235F |
|
40589-V08B6 |
S-ECD |
H69del, V70del, Y145del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H |
|
南非突变株 B.1.351 重要突变位点研究 |
||
|
货号 |
抗原 |
突变位点 |
|
40592-V08H59 |
RBD |
K417N |
|
40592-V08H84 |
RBD |
E484K |
|
40592-V08H85 |
RBD |
K417N, E484K, N501Y |
|
40591-V08H10 |
S1 |
K417N, E484K, N501Y, D614G |
|
40591-V08H13 |
S-NTD |
del(L242,A243,L244), L18F,D80A,D215G,R246I |
|
40591-V08H15 |
S1 |
del(L242,A243,L244), D215G, D80A, E484K, K417N, L18F, N501Y, R246I, D614G |
|
40589-V08B7 |
S |
del(L242,A243,L244), A701V, D215G, D80A, E484K, K417N, L18F, N501Y, R246I，D614G |
|
40588-V07E9 |
NP |
T205 |
|
巴西突变株 P.1 重要突变位点研究 |
||
|
货号 |
抗原 |
突变位点 |
|
40592-V08H86 |
RBD |
E484K,K417N,N501Y |
|
40591-V08H14 |
S1 |
L18F, T20N, P26S, D138Y, R190S, K417N, E484K, N501Y, D614G, H655Y |
|
40589-V08B8 |
S |
L18F, T20N, P26S, D138Y, R190S, K417N, E484K, N501Y, D614G, H655Y, T1027I |
|
40588-V07E11 |
NP |
P80R |
|
加州 B.1.429 突变株重要突变位点研究 |
||
|
货号 |
抗原 |
突变位点 |
|
40591-V08H17 |
S1 |
W152C, L452R, D614G |
|
40592-V08H28 |
RBD |
L452R |
|
|
||
|
货号 |
抗原 |
突变位点 |
|
40591-V08H9 |
S1 |
HV69-70 deletion, N439K, D614G |
|
40592-V08H12 |
RBD |
Y508H |
|
40592-V08H14 |
RBD |
N439K |
|
40592-V08H23 |
RBD |
Q414E |
|
40592-V08H28 |
RBD |
L452R |
|
40592-V08H30 |
RBD |
T478I |
|
40592-V08H31 |
RBD |
V483I |
|
40592-V08H35 |
RBD |
I472V |
|
40592-V08H4 |
RBD |
A435S |
|
40592-V08H5 |
RBD |
V483A |
|
40592-V08H50 |
RBD |
A475V |
|
40592-V08H83 |
RBD |
F490L |
新冠抗体产品
|
货号 |
分子 |
抗体类型 |
应用 |
|
40150-D001 |
S |
Chimera MAb |
ELISA, Neutralization |
|
40150-D002 |
S |
Chimera MAb |
ELISA, Neutralization |
|
40591-MM43 |
S |
Mouse MAb |
ELISA, Neutralization |
|
40592-MM57 |
S |
Mouse MAb |
ELISA, Neutralization |
|
40592-R0004 |
S |
Rabbit MAb |
ELISA, Neutralization |
|
40592-R001 |
S |
Rabbit MAb |
ELISA, Neutralization |
|
40592-MM55 |
S |
Mouse MAb |
ELISA, Non-Neutralization |
|
40592-R493 |
S |
Rabbit MAb |
ELISA, Non-Neutralization |
|
40591-MM42 |
S |
Mouse MAb |
WB,ELISA |
|
40150-R007 |
S |
Rabbit MAb |
ELISA |
|
40590-D001 |
S2 |
Chimera MAb |
ELISA |
|
40143-MM05 |
NP |
Mouse MAb |
ELISA, Neutralization |
|
40143-MM08 |
NP |
Mouse MAb |
WB,ELISA |
|
40143-R004 |
NP |
Rabbit MAb |
WB,ELISA |
|
40143-R040 |
NP |
Rabbit MAb |
WB,ELISA |
|
40143-R019 |
NP |
Rabbit MAb |
WB,ELISA |
|
40143-R001 |
NP |
Rabbit MAb |
WB,ELISA |
|
40588-R001 |
NP |
Rabbit MAb |
WB,ELISA,ELISA(Det) |
|
40588-MM123 |
NP |
Mouse MAb |
WB,ELISA,ELISA(Cap) |
|
40588-MM124 |
NP |
Mouse MAb |
WB,ELISA,ELISA(Cap), ELISA(Det) |
|
40588-R002 |
NP |
Rabbit MAb |
WB,ELISA,ELISA(Cap) |
|
40588-R004 |
NP |
Rabbit MAb |
WB,ELISA |
|
40588-R006-1 |
NP |
Rabbit MAb |
ELISA,ELISA(Det) |
新冠检测试剂盒、假病毒、基因产品
|
货号 |
名称 |
用途 |
|
KIT40588 |
Nucleoprotein / NP ELISA Kit |
双抗夹心法，定量检测样本中新冠Nucleocapsid蛋白水平 |
|
KIT40591 |
Spike ELISA Kit |
双抗夹心法，定量检测样本中新冠Spike S1蛋白水平 |
|
KIT40592 |
Spike RBD ELISA Kit |
双抗夹心法，定量检测样本中新冠Spike RBD蛋白水平 |
|
KIT001 |
Inhibitor Screening ELISA Kit |
ELISA竞争法，高通量、快速筛选出具有中和功能的抗体大分子或其他阻断剂 |
|
KIT002 |
Spike RBD Antibody Titer Assay Kit |
间接ELISA，检测样本中Spike RBD抗体滴度 |
|
KIT003 |
Spike S1 Antibody Titer Assay Kit |
间接ELISA，检测样本中Spike S1抗体滴度 |
|
KIT004 |
Spike S1+S2 ECD Antibody Titer Assay Kit |
间接ELISA，检测样本中Spike S1+S2 ECD抗体滴度 |
|
KIT005 |
Nucleocapsid/N Antibody Titer Assay Kit |
间接ELISA，检测样本中Nucleocapsid抗体滴度 |
|
KIT006 |
Spike RBD Antibody Titer Assay Kit (Mouse) |
间接ELISA，检测小鼠样本中Spike RBD抗体滴度 |
|
KIT007 |
Spike S1 Antibody Titer Assay Kit (Mouse) |
间接ELISA，检测小鼠样本中Spike S1抗体滴度 |
|
PSV001 |
Spike Pseudovirus |
新冠Spike假病毒，安全性高，替代真毒，用于中和抗体/血清检测等研究 |
|
PSV005 |
Spike (D614G) Pseudovirus |
新冠Spike (D614G)突变假病毒，安全性高，替代真毒，用于中和抗体/血清检测等研究 |
|
VG40771-UT |
Spike(B.1.1.7) Gene ORF cDNA clone expression plasmid (CodonOptimized) |
新冠英国突变株(B.1.1.7)Spike表达载体 |
|
VG40772-UT |
Spike(B.1.351) Gene ORF cDNA clone expression plasmid (CodonOptimized) |
新冠南非突变株(B.1.351)Spike表达载体 |
End
参考资料：
[1]https://doi.org/10.1101/2021.02.06.21251159
[2]https://doi.org/10.1101/2020.12.24.20248822
[3]https://doi.org/10.1101/2021.02.22.432357
[4]https://www.biorxiv.org/content/10.1101/2020.11.30.405472v1
[53]https://doi.org/10.1101/2021.02.18.431897