酵母杂交原理(供应SD/-Ade/-His/-Leu/-Met-Trp/-Ura with Agar和SD/-His/-Leu/-Met/-Ura with Agar等)
酵母双杂交是检测蛋白互作的常用手段之一。酵母双杂交系统由Fields和Song等首先在研究真核基因转录调控中建立。典型的真核生物转录因子，如GAL4、GCN4等都含有两个不同的结构域：DNA结合结构域(DNA-binding domain)和转录激活结构域(transcription-activating domain)。前者可识别DNA上的特异序列，并使转录激活结构域定位于所调节的基因的上游，转录激活结构域可同转录复合体的其他成分作用，启动它所调节的基因的转录。DNA-BD和AD单独分别作用并不能激活转录反应，但是当二者在空间上充分接近时，则呈现完整的GAL4转录因子活性并可激活UAS下游启动子，使启动子下游基因得到转录。
供应产品目录：
SD/-Ade with Agar10×0.5L
SD/-Ade/-His with Agar10×0.5L
SD/-Ade/-His/-Leu/-Met/-Trp with Agar10×0.5L
SD/-Ade/-His/-Leu/-Met-Trp/-Ura with Agar10×0.5L
SD/-Ade/-His/-Leu/-Trp with Agar10×0.5 L Pouches
SD/-Ade/-His/-Leu/-Trp/-Ura with Agar10×0.5L
SD/-Ade/-His/-Met with Agar10×0.5L
SD/-Ade/-His/-Trp with Agar10×0.5L Pouches
SD/-Ade/-Leu with Agar10×0.5L
SD/-Ade/-Leu/-His with Agar10×0.5L
SD/-Ade/-Leu/-Trp with Agar10×0.5L
SD/-Ade/-Trp with Agar10×0.5L Pouches
SD/-Arg/-His/-Leu/-Lys/-Ura with Agar10×0.5L
SD/-Cys/-Met/-Ura with Agar10×0.5L
SD/-Glu/-Ura with Agar10×0.5L
SD/-His with Agar10×0.5 L Pouches
SD/-His/-Leu with Agar10×0.5 L Pouches
SD/-His/-Leu/-Met/-Trp with Agar10×0.5 L Pouches
SD/-His/-Leu/-Met/-Ura with Agar10×0.5L
SD/-His/-Leu/-Trp with Agar10×0.5 L Pouches
SD/-His/-Leu/-Trp/-Ura with Agar10×0.5 L Pouches
SD/-His/-Leu/-Ura with Agar10×0.5 L Pouches
SD/-His/-Trp with Agar10×0.5L Pouches
SD/-His/-Trp/-Ura with Agar10×0.5 L Pouches
SD/-His/-Ura with Agar10×0.5 L Pouches
SD/-Leu with Agar10×0.5 L Pouches
SD/-Leu/-Ile/-Val with Agar10×0.5L
SD/-Leu/-Met/-Trp with Agar10×0.5L Pouches
SD/-Leu/-Met/-Trp/-Ura with Agar10×0.5L
SD/-Leu/-Trp with Agar10×0.5L Pouches
SD/-Leu/-Trp/-Ura with Agar10×0.5L Pouches