衰老及肠道菌群扰动对大脑免疫系统的影响 | 热心肠日报
多模型单细胞分析揭示在衰老和肠道菌群失调时大脑免疫图谱的可塑性
10.1016/j.celrep.2020.108438
12-01, Article
Abstract & Authors:展开
Abstract:收起
Phenotypic and functional plasticity of brain immune cells contribute to brain tissue homeostasis and disease. Immune cell plasticity is profoundly influenced by tissue microenvironment cues and systemic factors. Aging and gut microbiota dysbiosis that reshape brain immune cell plasticity and homeostasis has not been fully delineated. Using Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq), we analyze compositional and transcriptional changes of the brain immune landscape in response to aging and gut dysbiosis. Discordance between canonical surface-marker-defined immune cell types and their transcriptomes suggest transcriptional plasticity among immune cells. Ly6C+ monocytes predominate a pro-inflammatory signature in the aged brain, while innate lymphoid cells (ILCs) shift toward an ILC2-like profile. Aging increases ILC-like cells expressing a T memory stemness (Tscm) signature, which is reduced through antibiotics-induced gut dysbiosis. Systemic changes due to aging and gut dysbiosis increase propensity for neuroinflammation, providing insights into gut dysbiosis in age-related neurological diseases.
First Authors:
Samantha M Golomb
Correspondence Authors:
Siyuan Zhang
All Authors:
Samantha M Golomb,Ian H Guldner,Anqi Zhao,Qingfei Wang,Bhavana Palakurthi,Emilija A Aleksandrovic,Jacqueline A Lopez,Shaun W Lee,Kai Yang,Siyuan Zhang