如何选择PCR反应体系?
![](http://pic.ikafan.com/imgp/L3Byb3h5L2h0dHBzL2ltYWdlMTA5LjM2MGRvYy5jbi9Eb3dubG9hZEltZy8yMDIwLzEwLzI4MTcvMjA1NzM1OTU2XzFfMjAyMDEwMjgwNTMyMzEyMjguanBn.jpg)
2条引物扩增小于1kb的片段体系
![](http://pic.ikafan.com/imgp/L3Byb3h5L2h0dHBzL2ltYWdlMTA5LjM2MGRvYy5jbi9Eb3dubG9hZEltZy8yMDIwLzEwLzI4MTcvMjA1NzM1OTU2XzJfMjAyMDEwMjgwNTMyMzE3NzUuanBn.jpg)
备注:如果需要3条引物,则以1:1:1的比例,ddH2O减少,总体积不变。
4条引物扩增小于1kb的片段体系
![](http://pic.ikafan.com/imgp/L3Byb3h5L2h0dHBzL2ltYWdlMTA5LjM2MGRvYy5jbi9Eb3dubG9hZEltZy8yMDIwLzEwLzI4MTcvMjA1NzM1OTU2XzNfMjAyMDEwMjgwNTMyMzE5NjIuanBn.jpg)
LongAmp聚合酶的体系
![](http://pic.ikafan.com/imgp/L3Byb3h5L2h0dHBzL2ltYWdlMTA5LjM2MGRvYy5jbi9Eb3dubG9hZEltZy8yMDIwLzEwLzI4MTcvMjA1NzM1OTU2XzRfMjAyMDEwMjgwNTMyMzIxNjYuanBn.jpg)
短片段PCR 程序
![](http://pic.ikafan.com/imgp/L3Byb3h5L2h0dHBzL2ltYWdlMTA5LjM2MGRvYy5jbi9Eb3dubG9hZEltZy8yMDIwLzEwLzI4MTcvMjA1NzM1OTU2XzVfMjAyMDEwMjgwNTMyMzI0MDAuanBn.jpg)
备注:根据条带大小来确定延伸时间(诺唯赞P112-01是60s/kb,P222是20s/kb)
LongAmp聚合酶的PCR程序
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2条引物扩增小于1kb的片段体系
备注:如果需要3条引物,则以1:1:1的比例,ddH2O减少,总体积不变。
4条引物扩增小于1kb的片段体系
LongAmp聚合酶的体系
短片段PCR 程序
备注:根据条带大小来确定延伸时间(诺唯赞P112-01是60s/kb,P222是20s/kb)
LongAmp聚合酶的PCR程序