Plant Cell Environ.|上海交通大学石建新实验室揭示HSP70-16和VDAC3共同抑制冷胁迫下拟南芥种子萌发

在低温胁迫等不利条件下，脱落酸(ABA)在种子萌发过程中起着至关重要的作用。热休克蛋白70(HSP70)和电压依赖性阴离子通道(VDAC)蛋白都参与了 Arabidopsis 的冷应激反应。然而，它们在种子萌发过程中的 ABA 信号转导作用还不清楚。这里我们证明了拟南芥 HSP70-16和 VDAC3在低温胁迫条件下共同抑制种子萌发。在4 ° c 条件下，HSP70-16和 VDAC3均促进了胚乳 ABA 向胚的外排，从而抑制了种子萌发。HSP70-16与 VDAC3相互作用于细胞质膜和细胞核，HSP70-16与 VDAC3相互作用激活了 VDAC3离子通道的开放。

本研究建立了低温胁迫下 HSP70-16在种子萌发中的新功能，以及低温胁迫下 VDAC3活性与胚乳向胚转运 ABA 的可能关系。

本研究揭示了 HSP70-16与 VDAC3相互作用，促进 VDAC3离子通道的开放，从而影响胚乳向胚的 ABA 外流，从而对低温胁迫下的种子萌发起负向调节作用。

Abscisic acid (ABA) transport plays a crucial role in seed germination under unfavourable conditions such as cold stress. Both heat shock protein 70 (HSP70) and voltage-dependent anion channel (VDAC) protein are involved in cold stress responses in Arabidopsis. However, their roles in seed germination with regard to ABA signaling remain unknown. Here we demonstrated that Arabidopsis HSP70-16 and VDAC3 jointly suppress seed germination under cold stress conditions. At 4°C, both HSP70-16 and VDAC3 facilitated the efflux of ABA from the endosperm to the embryo and thus inhibited seed germination. HSP70-16 interacted with VDAC3 on the plasma membrane and in the nucleus, and the interplay between HSP70-16 and VDAC3 activated the opening of the VDAC3 ion channel. Our work established a novel function of HSP70-16 in seed germination under cold stress and a possible association of VDAC3 activity with ABA transportation from endosperm to embryo under cold stress conditions. This study reveals that HSP70-16 interacts with VDAC3 and facilitates the opening of the VDAC3 ion channel, which influences ABA efflux from endosperm to embryo, thus negatively regulates seed germination under cold stress.

https://doi.org/10.1111/pce.14138

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