Plant J: 广西农业科学院水稻研究所邓国富团队在水稻细菌性条斑病抗性研究中取得新进展
细菌性条斑病是水稻的主要细菌性病害之一。利用宿主遗传抗性已成为防治 BLS 的重要策略之一。然而，只有少数几个抗性基因被确定为特征。在此之前，一个隐性细菌条斑病抗性基因 bls1被粗略定位在第6染色体上。在这里，我们进一步描述了 bls1到一个跨越4个基因的21kb 区域。基因分析证实，编码有丝分裂原活化蛋白激酶(OsMAPK6)的基因是等位基因 BLS1和 BLS1的靶基因。BLS1的过表达减弱，而 BLS1的低表达增加了对水稻白叶枯病菌的抗性。稻瘟病菌(Oryzicola (Xoc)菌株 JZ-8。但是，BLS1的过表达和 BLS1的低表达均可增加无种族特异性的宽谱抗性。
这些结果表明，BLS1和 BLS1对 Xoc 株 JZ-8的小种特异性抗性具有负向调节作用，而对宽谱抗性具有正向和负向调节作用。亚细胞定位显示 OsMAPK6定位于细胞核内。已知调解对 Xoc 的抗性的 RGA4是 OsMAPK6的潜在目标。BLS1的过表达和 BLS1的低表达显示水杨酸的增加和防御相关基因的诱导表达，同时增加了抗广谱性。
此外，bls1的低表达使茉莉酸和脱落酸增加，同时对 Xoc 菌株 JZ-8的抗性增强。本研究为进一步了解水稻对 BLS 的抗性提供了新的视角，有助于水稻抗寄主品种的培育。
Bacterial leaf streak (BLS) is one of the most major bacterial diseases of rice. Utilization of host genetic resistance has become one of the most important strategies to control BLS. However, only a few resistance genes have been characterized. Previously, a recessive bacterial leaf streak resistance gene bls1 was roughly mapped on the chromosome 6. Here, we further delineated the bls1 to a 21 kb region spanning 4 genes. Genetic analysis confirmed the gene encoding a mitogen-activated protein kinase (OsMAPK6) is the target of the allelic genes BLS1 and bls1. Overexpression of BLS1 weakened, while low expression of bls1increased the resistance to the specific Xanthomonas oryze pv. Oryzicola (Xoc) strain JZ-8. However, both overexpression of BLS1 and low expression of bls1 could increase the no-race specific broad-spetrum resistance. These results indicate BLS1 and bls1 negatively regulate the race specific resistance to Xoc strain JZ-8, but positively and negatively control the broad-spetrum resistance, respectively. Subcellular localization demonstrated that OsMAPK6 was localized in the nucleus. RGA4 that is known to mediate the resistance to Xoc is the potential target of OsMAPK6. Overexpression of BLS1 and low expression of bls1 showed increase of salicylic acid and induced expression of defense-related genes, simultaneously increasing the broad-spetrum resistance. Moreover, low expression of bls1 showed increase of jasmonic acid and abscisic acid, in company with an increase resistance to Xoc strain JZ-8. Collectively, our study provides a new insight into the understanding of BLS resistance and facilitates the development of rice host-resistant cultivars.
10.1111/tpj.15368
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