CRISPR / Cas9介导的MaACO1基因编辑可延长香蕉果实的保质期

在自然成熟的条件下,野生型水果收获后18-21天产生了大量的乙烯(图1j),而在MaACO1敲除的品系中,乙烯的生产被强烈延迟和减少(图1j) )。在乙烯利处理下,野生型果实和MaACO1敲除品系之间的乙烯生产表现出相似的趋势,但是在野生型品系中,与MaACO1敲除品系相比,第3天产生了更多的乙烯(图1k) 。 “总而言之,我们使用CRISPR / Cas9系统创建了几种具有不同编辑模式的MaACO1编辑植物突变果实在自然成熟条件下表现出减少的乙烯合成并延长了保质期。此外,MaACO1编辑植株的果实还对乙烯利和乙烯利敏感。 乙烯利处理后可正常成熟。此外,编辑后的MaACO1的品系的营养生长,生命周期和果实品质与野生型植物相当,但高度和产量略低。”

"Under natural ripening conditions, high amounts of ethylene were produced 18–21 days post-harvest in wild-type fruits (Figure 1j), whereas, in the MaACO1-disrupted line, ethylene production was strongly delayed and reduced (Figure 1j). Under ethephon treatment, ethylene production exhibited a similar trend between the wild-type fruits and MaACO1-disrupted lines, but in the wild-type line, more ethylene was produced at day 3 compared with the MaACO1-disrupted line (Figure 1k).

"In summary, we created several MaACO1-disrupted plants with different editing patterns using the CRISPR/Cas9 system. The mutant fruits exhibited reduced ethylene synthesis and extended shelf life under natural ripening conditions. Moreover, MaACO1-disrupted fruit was also sensitive to ethephon and ripened normally after ethephon treatment. In addition, the vegetative growth, lifecycle, and fruit quality of the MaACO1-disrupted line were comparable to those of wild-type plants except for slightly lower height and yield."

https://onlinelibrary.wiley.com/doi/10.1111/pbi.13534

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