Plant J|拟南芥花阻遏物TEMPRANILLO1和2通过调节激素成分和光保护来调节耐盐性
植物特异性RAV转录因子家族的成员调节一些发育和生理过程。在拟南芥模式植物中，RAV-templanillo-1（TEM1）和TEM2控制着重要的阶段变化，如幼体到成虫和营养体到生殖的转变。除了它们在植物发育中已知的调节功能外，对过表达TEM1的转基因植物的转录组学分析也揭示了与非生物胁迫反应相关。GO分析发现胁迫相关基因比例较高。
因此，为了研究这些依赖TEM的转录组变化的生物学相关性，并阐明TEM是否参与了植物生长对盐胁迫的响应，我们分析了TEM获得和功能丧失突变体在不同发育阶段受到轻度和高盐胁迫的行为。TEM高表达植株对盐胁迫敏感，而tem1-tem2双突变体表现出较强的耐盐性。准确地说，tem1-tem2突变体在盐胁迫条件下比野生型植物发芽开花快。此外，tem1-tem2植株在盐诱导的叶片衰老过程中表现出延迟，这可能是茉莉酸生物合成基因下调的结果。tem1-tem2突变体除具有较短的生命周期和延缓衰老外，还具有较高的抗氧化代谢产物α-生育酚（一种重要的抗氧化代谢物）的积累和光合色素的缓慢降解，因而更适合于承受氧化应激。
综上所述，我们的研究表明TEM在适应性生长中具有新的和关键的作用，因为它们调节植物的发育，以响应环境变化，如增加土壤盐分。
Members of the plant specific RAV family of transcription factors regulate several developmental and physiological processes. In the model plant Arabidopsis thaliana, the RAV TEMPRANILLO 1 (TEM1) and TEM2 control important phase changes such as the juvenile to adult and the vegetative to reproductive transitions. Besides their known regulatory function in plant development, a transcriptomics analysis of transgenic plants overexpressing TEM1 also revealed overrepresentation of Gene Ontology (GO) categories related to abiotic stress responses. Therefore, to investigate the biological relevance of these TEM‐dependent transcriptomic changes and elucidate whether TEMs contribute to the modulation of plant growth in response to salinity, we analyzed the behavior of TEM gain and loss of function mutants subjected to mild and high salt stresses at different development stages. With respect to increasing salinity, TEM overexpressing plants were hypersensitive whereas the tem1 tem2 double mutants were more tolerant. Precisely, tem1 tem2 mutants germinated and flowered faster than the wild‐type plants under salt stress conditions. Also, tem1 tem2 plants showed a delay in salt‐induced leaf senescence, possibly as a consequence of downregulation of jasmonic acid biosynthesis genes. Besides a shorter life cycle and delayed senescence, tem1 tem2 mutants appeared to be better suited to withstand oxidative stress as they accumulated higher levels of α‐tocopherol (an important antioxidant metabolite) and displayed a slower degradation of photosynthetic pigments. Taken together, our studies suggest novel and crucial roles for TEM in adaptive growth as they modulate plant development in response to environmental changes such as increasing soil salinity.
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