外国的生信技能树转录组教程
从实验设计,实验操作细节,到测序数据一条龙分析都面面俱到,本来是想搜索看看他们有没有出配套教学视频在YouTube,虽然没有找到他们的频道,但是无意中找到了一个类似于我们生信技能树的平台:
![](http://n4.ikafan.com/assetsj/blank.gif)
https://www.youtube.com/channel/UCKbkfKk65PZyRCzUwXOJung
每年都有七八个视频专题,高产似母猪!随便进入其中一个:
![](http://n4.ikafan.com/assetsj/blank.gif)
真的是可以跟我们生信技能树媲美了!
目录如下:
Module 0 - Introduction and Cloud Computing
Authors
Citation and Supplementary Materials
Syntax
Intro to AWS Cloud Computing
Logging into Amazon Cloud
Unix Bootcamp
Environment
Resources
Module 1 - Introduction to RNA sequencing
Installation
Reference Genomes
Annotations
Indexing
RNA-seq Data
Pre-Alignment QC
Module 2 - RNA-seq Alignment and Visualization
Adapter Trim
Alignment
IGV
Alignment Visualization
Alignment QC
Module 3 - Expression and Differential Expression
Expression
Differential Expression
DE Visualization
Kallisto for Reference-Free Abundance Estimation
Module 4 - Isoform Discovery and Alternative Expression
Reference Guided Transcript Assembly
de novo Transcript Assembly
Transcript Assembly Merge
Differential Splicing
Splicing Visualization
Module 5 - De novo transcript reconstruction
De novo RNA-Seq Assembly and Analysis Using Trinity
Module 6 - Functional Annotation of Transcripts
Functional Annotation of Assembled Transcripts Using Trinotate
Appendix
Saving Your Results
Abbreviations
Lectures
Practical Exercise Solutions
Integrated Assignment
Proposed Improvements
AWS Setup
写在后面
如果你在海外,那毫无疑问,推荐你看他们的教程和视频咯,但是很有可能,你在国内,YouTube访问有困难,或者英文理解不到位,那就只能说看我们生信技能树教程啦!
![](http://n4.ikafan.com/assetsj/blank.gif)
转录组从这3个开始哈,而且还有3个配套习题!
初级10 个题目,尽量根据参考代码理解及完成:http://www.bio-info-trainee.com/3793.html
中级要求是:http://www.bio-info-trainee.com/3750.html
高级要求是完成20题:http://www.bio-info-trainee.com/3415.html
统计专题 30题:http://www.bio-info-trainee.com/4385.html
可视化专题30题:http://www.bio-info-trainee.com/4387.html
最低要求是完成我的 linux 20题 http://www.bio-info-trainee.com/2900.html
其次完成生物信息学数据格式的习题(blast/blat/fa-fq/sam-bam/vcf/bed/gtf-gff),收集这些格式的说明书。
fasta和fastq格式文件的shell小练习 http://www.bio-info-trainee.com/3575.html
sam和bam格式文件的shell小练习 http://www.bio-info-trainee.com/3578.html
VCF格式文件的shell小练习 http://www.bio-info-trainee.com/3577.html
http://www.bio-info-trainee.com/3920.html