补充支链氨基酸对肌肉损伤所致血浆氨基酸浓度和肌肉力能学变化的影响：随机安慰剂对照试验

Clin Nutr. 2016 Feb;35(1):83-94.

Effects of branched-chain amino acids supplementation on both plasma amino acids concentration and muscle energetics changes resulting from muscle damage: A randomized placebo controlled trial.

Fouré A, Nosaka K, Gastaldi M, Mattei JP, Boudinet H, Guye M, Vilmen C, Le Fur Y, Bendahan D, Gondin J.

Aix-Marseille Université, CNRS, Centre de Résonance Magnétique Biologique et Médicale (CRMBM), UMR CNRS 7339, 13385 Marseille, France; Edith Cowan University, School of Exercise and Health Sciences, Center for Exercise and Sports Science Research, Joondalup, WA 6027, Australia; Aix Marseille Université, INSERM, INRA, Nutrition, Obésité et Risque Thrombotique (NORT), UMR INSERM U1062 - INRA 1260, 13385 Marseille, France; APHM, Hôpital Sainte Marguerite, Service de Rhumatologie, 13009 Marseille, France; APHM, Hôpitaux de La Timone, Pôle d'imagerie médicale, Centre d'Exploration Métabolique par Résonance Magnétique (CEMEREM), 13385 Marseille, France.

BACKGROUND & AIMS: Branched-chain amino acids promote muscle-protein synthesis, reduce protein oxidation and have positive effects on mitochondrial biogenesis and reactive oxygen species scavenging. The purpose of the study was to determine the potential benefits of branched-chain amino acids supplementation on changes in force capacities, plasma amino acids concentration and muscle metabolic alterations after exercise-induced muscle damage.

METHODS: (31)P magnetic resonance spectroscopy and biochemical analyses were used to follow the changes after such damage. Twenty six young healthy men were randomly assigned to supplemented branched-chain amino acids or placebo group. Knee extensors maximal voluntary isometric force was assessed before and on four days following exercise-induced muscle damage. Concentrations in phosphocreatine [PCr], inorganic phosphate [Pi] and pH were measured during a standardized rest-exercise-recovery protocol before, two (D2) and four (D4) days after exercise-induced muscle damage.

RESULTS: No significant difference between groups was found for changes in maximal voluntary isometric force (-24% at D2 and -21% at D4). Plasma alanine concentration significantly increased immediately after exercise-induced muscle damage (+25%) in both groups while concentrations in glycine, histidine, phenylalanine and tyrosine decreased. No difference between groups was found in the increased resting [Pi] (+42% at D2 and +34% at D4), decreased resting pH (-0.04 at D2 and -0.03 at D4) and the slower PCr recovery rate (-18% at D2 and -24% at D4).

CONCLUSIONS: The damaged muscle was not able to get benefits out of the increased plasma branched-chain amino acids availability to attenuate changes in indirect markers of muscle damage and muscle metabolic alterations following exercise-induced muscle damage.

KEYWORDS: Double blind randomized placebo controlled trial; Exercise induced-muscle damage; Magnetic resonance spectroscopy

PMID: 25886707

DOI: 10.1016/j.clnu.2015.03.014