PJ|西北农林园艺系王跃进和徐炎共同通讯揭示VvHDZ28正调控汤普森无核葡萄种子败育过程中的水杨酸生物合成

葡萄无籽是消费者最喜欢的特性之一。然而，葡萄无籽的分子机制仍然不清楚。在这里，我们观察到小球状胚和球状胚在黑比诺和汤普森无核开花后20至30天（DAF）。在40～50daf期间，我们观察到黑比诺的鱼雷胚和子叶胚，而汤普森无核葡萄的败育胚和胚乳。

因此，对汤普森无籽和黑比诺在这些阶段的种子进行了RNA-Seq分析。共鉴定出6442个差异表达基因。其中，参与水杨酸生物合成的基因VvEDS1和VvSARD1在汤普森无核葡萄中的表达高于黑比诺。

此外，汤普森无籽葡萄的内源水杨酸含量至少是黑比诺的5倍。VvEDS1和VvSARD1中H3K27的三甲基化增加可能与黑比诺中SA含量较低有关。

我们还证明VvHDZ28正调控VvEDS1的表达。此外，VvHDZ28的过度表达导致番茄无核果实和SA含量增加。

我们的结果揭示了SA和VvHDZ28在无核葡萄中的反馈调节的潜在作用。

Seedlessness in grapes is one of the features most appreciated by consumers. However, the mechanisms underlying seedlessness in grapes remain obscure. Here, we observe small globular embryos and globular embryos in Pinot Noir and Thompson Seedless from 20 to 30 days after flowering (DAF). From 40 to 50 DAF, we observe torpedo embryos and cotyledon embryos in Pinot Noir but aborted embryos and endosperm in Thompson Seedless. Thus, RNA‐Seq analyses of seeds at these stages from Thompson Seedless and Pinot Noir were performed. A total of 6442 differentially expressed genes were identified. Among these, genes involved in SA biosynthesis, VvEDS1 and VvSARD1, were more highly expressed in Thompson Seedless than in Pinot Noir. Moreover, the content of endogenous SA is at least five‐times higher in Thompson Seedless than in Pinot Noir. Increased trimethylation of H3K27 of VvEDS1 and VvSARD1 may be correlated with lower SA content in Pinot Noir. We also demonstrate that VvHDZ28 positively regulates the expression of VvEDS1. Moreover, over‐expression of VvHDZ28 results in seedless fruit and increased SA contents in Solanum lycopersicum. Our results reveal the potential role of SA and feedback regulation of VvHDZ28 in seedless grapes.Seedlessness in grapes is one of the features most appreciated by consumers. However, the mechanisms underlying seedlessness in grapes remain obscure. Here, we observe small globular embryos and globular embryos in Pinot Noir and Thompson Seedless from 20 to 30 days after flowering (DAF). From 40 to 50 DAF, we observe torpedo embryos and cotyledon embryos in Pinot Noir but aborted embryos and endosperm in Thompson Seedless. Thus, RNA‐Seq analyses of seeds at these stages from Thompson Seedless and Pinot Noir were performed. A total of 6442 differentially expressed genes were identified. Among these, genes involved in SA biosynthesis, VvEDS1 and VvSARD1, were more highly expressed in Thompson Seedless than in Pinot Noir. Moreover, the content of endogenous SA is at least five‐times higher in Thompson Seedless than in Pinot Noir. Increased trimethylation of H3K27 of VvEDS1 and VvSARD1 may be correlated with lower SA content in Pinot Noir. We also demonstrate that VvHDZ28 positively regulates the expression of VvEDS1. Moreover, over‐expression of VvHDZ28 results in seedless fruit and increased SA contents in Solanum lycopersicum. Our results reveal the potential role of SA and feedback regulation of VvHDZ28 in seedless grapes.

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