七氟醚对人肿瘤细胞生长和凋亡的影响
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Differential effects of sevoflurane on the growth and apoptosis of human cancer
背景与目的
目前关于七氟醚对癌细胞致癌特性影响的研究相互矛盾。本研究深入探讨七氟醚暴露对多种来源癌细胞的影响。
方 法
将一系列癌细胞株暴露于1%七氟醚中2-8小时，然后与未经暴露的癌细胞株比较，评估其增殖、基质凝胶浸润和凋亡细胞死亡。分别采用Coulter计数法和Boyden室法进行细胞增殖和基质凝胶侵袭试验。用Annexin V和7-AAD染色细胞，荧光流式细胞仪检测细胞凋亡。此外，用免疫印迹法检测凋亡标志物caspase-3蛋白的表达。
结 果
七氟醚暴露后，8种肿瘤细胞株（NCI-H1299、MDA-MB-231、HCT116、DLD-1、HT29和RKO）中的6种细胞增殖明显增强。与此相反，七氟醚抑制了后两种癌细胞系A549和MCF-7以及非癌细胞系MCF10A的增殖。对四种癌细胞系进行的细胞生物学实验表明，七氟醚暴露后，癌细胞增殖加速而非减弱，其与增强基质侵袭和抑制凋亡有关。
结 论
七氟醚增强或抑制细胞增殖和基质侵袭对不同癌细胞系影响不一，且七氟醚诱导癌细胞凋亡可能与七氟醚暴露后细胞增殖增强有关。
原始文献摘要
Hirai T, Konishi Y, Mizuno S；Differential effects of sevoflurane on the growth and apoptosis of human cancer；J Anesth 2020 Feb;34(1)；PMID：31667585
PURPOSE:There have been contradictory findings regarding the effects of sevoflurane on the oncogenic properties of cancer cells. This study was conducted to gain insights into the fundamental rules governing the differential effects of sevoflurane exposure on various cancer cells derived from multiple origins.
METHODS:A series of cancer cell lines were exposed to 1% (v/v) sevoflurane for 2-8 h and then assessed for their proliferation, Matrigel invasion, and apoptotic cell death, in comparison with their untreated counterparts. Cell proliferation and Matrigel invasion assays were performed using Coulter counter and Boyden chamber techniques, respectively. Apoptosis was evaluated by staining cells with Annexin V and 7-AAD followed by fluorescence flow cytometry. In addition, the expression of cleaved caspase-3 protein, another marker of apoptosis, was assessed using immunoblotting.
RESULTS:Proliferation was significantly enhanced after sevoflurane exposure in six of eight cancer cell lines (NCI-H1299, MDA-MB-231, HCT116, DLD-1, HT29, and RKO). In contrast, sevoflurane attenuated proliferation in the last two cancer cell lines, A549 and MCF-7, as well as in the non-cancerous MCF10A cell line. Cell biological assays using four cancer cell lines demonstrated that accelerated but not attenuated cancer cell proliferation after sevoflurane exposure is associated with enhanced Matrigel invasion and suppressed apoptosis.
CONCLUSION:Sevoflurane augmented or hampered cell proliferation and Matrigel invasion depending on the cancer cell line examined. Loss of sevoflurane-induced apoptosis occurring in cancer cell lines is likely to be correlated with their enhanced proliferation after sevoflurane exposure.
麻醉学文献进展分享
贵州医科大学高鸿教授课题组
翻译：王贵龙 编辑：冯玉蓉 审校：王贵龙