SWEET转运蛋白及其在茶树中的潜在功能
第一作者
Lan Jiang
第一单位
皖南医学院弋矶山医院
通讯作者
Xi Zhu and Jianke Yang
Abstract
背景+问题：Tea (Camellia sinensis) is an important economic beverage crop. Its flowers and leaves could be used as healthcare tea for its medicinal value. SWEET proteins were recently identified in plants as sugar transporters, which participate in diverse physiological processes, including pathogen nutrition, seed filling, nectar secretion, and phloem loading. Although SWEET genes have been characterized and identified in model plants, such as Arabidopsis thaliana and Oryza sativa, there is very little knowledge of these genes in C. sinensis.
主要研究：In the present study, 26 CsSWEETs in C. sinensis were identified, and their gene structures, phylogenetic, microsynteny, and expression were analyzed.
结果1：28 CsSWEETs were identified in C. sinensis and further phylogenetically divided into four subfamilies with A. thaliana. These identified CsSWEETs contained seven transmembrane helixes (TMHs) which were generated by an ancestral three-TMH unit with an internal duplication experience. Microsynteny analysis revealed that the large-scale duplication events were the main driving forces for members from CsSWEET family expansion in C. sinensis.
结果2：The expression profiles of the 28 CsSWEETs revealed that some genes were highly expressed in reproductive tissues. Among them, CsSWEET1a might play crucial roles in the efflux of sucrose, and CsSWEET17b could control fructose content as a hexose transporter in C. sinensis. Remarkably, CsSWEET12 and CsSWEET17c were specifically expressed in flowers, indicating that these two genes might be involved in sugar transport during flower development. The expression patterns of all CsSWEETs were differentially regulated under cold and drought treatments.
结论：This work provided a systematic understanding of the members from the CsSWEET gene family, which would be helpful for further functional studies of CsSWEETs in C. sinensis.
摘 要
茶是重要的经济饮料作物。它的花朵和叶子因其药用价值可以用作保健茶。SWEET蛋白最近在植物中被鉴定为糖转运蛋白，参与多种生理过程，包括病原体营养，种子充实，花蜜分泌和韧皮部负载。尽管已在模型植物（如拟南芥和水稻）中对SWEET基因进行了表征和鉴定，但在茶树中对这些基因的了解甚少。在这项研究中，在茶树中鉴定出28个CsSWEET，并进一步在系统发育上分为四个亚类。这些确定的CsSWEET包含七个跨膜螺旋（TMH），它们是由一个具有内部复制经验的祖先三个TMH单元生成的。显微同步分析表明，大规模复制事件是茶树CsSWEET家族扩张成员的主要驱动力。28个CsSWEET的表达谱表明，某些基因在生殖组织中高度表达。其中，CsSWEET1a可能在蔗糖的外流中起关键作用，而CsSWEET17b可以控制果糖的含量作为茶树的己糖转运蛋白。值得注意的是，CsSWEET12和CsSWEET17c在花中特异性表达，表明这两个基因可能与花发育过程中的糖转运有关。在寒冷和干旱条件下，所有CsSWEET的表达模式均受到差异调节。这项工作为CsSWEET基因家族的成员提供了系统的了解，这将有助于进一步研究茶树中CsSWEET的功能。
DOI: https://doi.org/10.3389/fgene.2021.655843
Journal: Fontiers in Genetics
Impact Factor：3.258
Published date: 31 March 2021
END
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