低温对绿色和白化茶树叶片中类胡萝卜素生物合成的影响
第一作者
Yazhuo Yang
第一单位
南京农大
通讯作者
Jing Zhuang
Abstract
背景+问题：Tea plant is one of the most important economic crops worldwide, and it has a positive effect on human health. The low temperature in late autumn and early/late spring cold often cause damage to the normal physiological activities of tea plant, thereby reducing the quality and yield of tea. As one of the essential pigment of higher plant, carotenoids play an important role in the physiological activities of higher plant. In addition, as an important precursor of tea aroma volatiles, carotenoids are the important factors involved in the formation of tea quality.
主要研究：In this study, the main carotenoids, lutein and β-carotene, in leaves of common green tea cultivar 'Longjing 43′ and albino tea cultivar 'White leaf No.1′ were detected after low temperature treatment at 4 ℃.
结果1：The results showed that the contents of lutein and β-carotene in 'Longjing 43′ were higher than that in 'White leaf No.1′, respectively. The chlorophyll content in two tea cultivars decreased and was significant positive correlation with two kinds of carotenoid content at low temperature.
结果2：Under low temperature, the genes related to the biosynthesis pathway of methylerythritol 4-phosphate (MEP) and carotenoids all showed varying degrees of response with the changes of lutein and β-carotene content. The relative expression levels of CsDXS1, CsPSY1 and CsCHXB were up-regulated and higher than other related genes. Expression analysis of carotenoids biosynthesis genes and its correlation with carotenoids accumulation confirmed the regulatory role of structural genes in tea plant at low temperature. Promoter prediction results showed that the CsDXR, CsHDS1 and CsCHXB contain low-temperature response element LTR.
结论：This study provides reference for further study on the dynamic changes of carotenoids during the growth of tea plant.
摘 要
茶树是全球最重要的经济作物之一，对人类健康具有积极作用。秋末和初春寒冷的低温往往会破坏茶树的正常生理活动，从而降低茶的质量和产量。类胡萝卜素作为高等植物的必需色素之一，在高等植物的生理活动中起着重要的作用。另外，类胡萝卜素作为茶香气挥发物的重要前体，是影响茶品质形成的重要因素。本研究在4℃的低温处理下，检测到普通绿茶品种“龙井43”和白化茶品种“白叶1号”叶片中的主要类胡萝卜素，叶黄素和β-胡萝卜素。结果表明，龙井43号的叶黄素和β-胡萝卜素含量分别高于白叶1号。两个茶叶品种的叶绿素含量在低温下均下降，并且与两种类胡萝卜素含量呈显着正相关。在低温下，随着叶黄素和β-胡萝卜素含量的变化，与4-磷酸赤藓糖醇（MEP）和类胡萝卜素的生物合成途径相关的基因均表现出不同程度的响应。CsDXS1，CsPSY1和CsCHXB的相对表达水平被上调并且高于其他相关基因。类胡萝卜素生物合成基因的表达分析及其与类胡萝卜素积累的相关性证实了低温下茶树中结构基因的调控作用。启动子预测结果表明，CsDXR，CsHDS1和CsCHXB包含低温响应元件LTR。该研究为进一步研究茶树生长过程中类胡萝卜素的动态变化提供了参考。
DOI: https://doi.org/10.1016/j.scienta.2021.110164
Journal: Scientia Horticulturae
Impact Factor：2.769
Published date: 14 April 2021
科学技术史上的今天
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