活死细胞染色研究案例,附文献~
关于活死细胞染色的研究,Calcein AM和碘化丙啶(PI)这两种活死细胞染色试剂盒:Calcein AM是一种疏水化合物,容易渗透完整的活细胞,进入细胞后被酯酶水解产生强烈荧光,同时它能完整的保存在细胞细胞质中,酯酶活性与活细胞的数量成正比。对于具有完整膜的活细胞,DNA染料PI是J性的无法渗透细胞膜,它仅能结合死细胞的DNA,一起来看下检测结果(如图):
1、22789细胞成像分析——皂苷诱导Jurkat 细胞死亡的影响
2、22789细胞成像分析——90%活细胞,0%活细胞(固定细胞)以及两者混合的对比
3、22788染色HeLa细胞的荧光图像
活死细胞染色部分产品引文赏析:
1.Fabrication of Tunable 3D Cellular Structures in High Volume Using Magnetic Levitation Guided Assembly(Cat# 22789)
2.Resminostat, a histone deacetylase inhibitor, circumvents tolerance to EGFR inhibitors in EGFR-mutated lung cancer cells with BIM deletion polymorphism(Cat# 22789)
3.T cell activation profiles distinguish hemophagocytic lymphohistiocytosis and early sepsis(Cat# 22789)
4.Effect of copper nanoparticles on physico-chemical properties of chitosan and gelatin-based scaffold developed for skin tissue engineering application(Cat# 22789)
5.Ultrasound and microbubbles (USMB) potentiated doxorubicin penetration and distribution in 3D breast tumour spheroids(Cat# 22788)