Plant Biotechnol. J|茉莉酸和脱落酸激活的AaGSW1-AaTCP15/AaORA转录级联促进青蒿素的生物合成
青蒿素是一种广泛用于疟疾治疗的倍半萜内酯，是在药用植物青蒿中发现的。茉莉酸（JA）和脱落酸（ABA）通过调节因子有效地调节青蒿素的生物合成。然而，通过下游转录因子（TFs）的相关调控网络将JA和ABA信号与青蒿素生物合成联系起来的机制仍然是个谜。
在这里我们报告了AaTCP15，这是一种JA和ABA双重反应的teosinte分支的1 / cycloidea / proliferating（TCP）TF，它通过直接结合并激活DBR2和ALDH1的启动子（这两个基因编码）对JA和ABA诱导的青蒿素的生物合成至关重要。青蒿素生物合成所需的酶。此外，青蒿素生物合成的另一种正调节剂AaORA响应JA和ABA，与AaTCP15相互作用并增强其反式激活活性，并同时激活AaTCP15转录本。因此，它们形成了一个AaORA-AaTCP15模块来协同激活DBR2，这是青蒿素生物合成的关键基因。
更重要的是，AaTCP15表达被多种报道的促进青蒿素生物合成的JA和ABA响应性TF激活。其中，AaGSW1作用于JA和ABA信号的联系，以激活青蒿素的生物合成途径，并与AaORA启动子分开直接结合并激活aTCP15启动子，这进一步促进了AaGSW1-AaTCP15 / AaORA调节模块的形成以整合JA和ABA介导的青蒿素生物合成。我们的结果建立了AaGSW1-AaTCP15 / AaORA模块的多层调控网络，以通过JA和ABA信号调控青蒿素的生物合成，并为将来的研究探索与植物特定代谢物相关的TCP基因的特殊转录调控模块提供了有趣的途径。
Artemisinin, a sesquiterpene lactone widely used in malaria treatment, was discovered in the medicinal plant Artemisia annua. The biosynthesis of artemisinin is efficiently regulated by jasmonate (JA) and abscisic acid (ABA) via regulatory factors. However, the mechanisms linking JA and ABA signalling with artemisinin biosynthesis through an associated regulatory network of downstream transcription factors (TFs) remain enigmatic. Here we report AaTCP15, a JA and ABA dual‐responsive teosinte branched1/cycloidea/proliferating (TCP) TF, which is essential for JA and ABA‐induced artemisinin biosynthesis by directly binding to and activating the promoters of DBR2 and ALDH1, two genes encoding enzymes for artemisinin biosynthesis. Furthermore, AaORA, another positive regulator of artemisinin biosynthesis responds to JA and ABA, interacts with and enhances the transactivation activity of AaTCP15 and simultaneously activates AaTCP15 transcripts. Hence, they form an AaORA‐AaTCP15 module to synergistically activate DBR2, a crucial gene for artemisinin biosynthesis. More importantly, AaTCP15 expression is activated by the multiple reported JA and ABA‐responsive TFs that promote artemisinin biosynthesis. Among them, AaGSW1 acts at the nexus of JA and ABA signalling to activate the artemisinin biosynthetic pathway and directly binds to and activates the AaTCP15 promoter apart from the AaORA promoter, which further facilitates formation of the AaGSW1‐AaTCP15/AaORA regulatory module to integrate JA and ABA‐mediated artemisinin biosynthesis. Our results establish a multilayer regulatory network of the AaGSW1‐AaTCP15/AaORA module to regulate artemisinin biosynthesis through JA and ABA signalling, and provide an interesting avenue for future research exploring the special transcriptional regulation module of TCP genes associated with specialized metabolites in plants.
链接：
Jasmonate‐ and abscisic acid‐activated AaGSW1‐AaTCP15/AaORA transcriptional cascade promotes artemisinin biosynthesis in Artemisia annua10.1111/pbi.13561
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