使用丙泊酚和依托咪酯麻醉对心血管组织miRNA表达的影响：不同的表达谱？
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Effects of propofol and etomidate anesthesia on cardiovascular miRNA expression: the different profiles?
背景与目的
静脉麻醉药丙泊酚和依托咪酯对循环系统的影响存在显著差异; 然而，其对心血 管系统中miRNA表达谱的影响尚不清楚。本研究的目的是探讨这两种麻醉药物对心脏和血 管组织中miRNA表达谱的影响。
方 法
将大鼠随机分成丙泊酚组和依托咪酯组。在整个麻醉过程中确保大鼠的氧气供应 并保留自主呼吸。诱导后3小时获取心脏和胸主动脉组织。通过微阵列4.0分析检测心血 管组织中miRNA的表达谱。选择12种具有代表性的miRNA进行qRT-PCR验证，并使用生物信 息学方法预测其靶基因。
结 果
微阵列分析显示与依托咪酯组比较，丙泊酚组心脏组织中有16种miRNA存在差异表 达（10种上调和6种下调），而血管组织中有25种miRNA存在差异表达（10种上调，15种 下调）。通过qRT-PCR验证12种具有代表性的miRNA后，结果显示两组心脏组织中miRNA的 表达无显著差异，但血管组织中有5种miRNA的表达存在显著差异。生物信息学分析预测 该5种存在差异表达miRNA的靶基因与化学突触信号传导途径相关。
结 论
丙泊酚和依托咪酯对心血管组织中miRNA的表达存在不同的影响，需要进一步研究 来表明这些miRNA的差异表达对心血管系统功能的影响。
原始文献摘要
Yao Y1, Yang N2, Effects of propofol and etomidate anesthesia on cardiovascular miRNA expression: the different profiles? BM C Anesthesiol. 2018 Oct 24;18(1):149.
BACKGROUND:
The effects of the intravenous anesthetics propofol and etomidate on circulation are significantly different; however, their differing effects on miRNA expression in the cardiovascular system are not clearly understood. The purpose of this study is to investigate the effects of these two anesthetics on miRNA expression profiles in the heart and blood vessels.
METHODS:
Rats were randomly divided into a propofol group and an etomidate group. Spontaneous breathing was maintained throughout the anesthesia process and the rats' oxygen supply was ensured. Heart and thoracic aorta tissue was harvested 3 h after induction. The expression profiles of cardiovascular miRNAs were detected by microarray 4.0 analysis. Twelve representative miRNAs were selected for qRT-PCR validation, and their target genes were predicted using bioinformatics methods.
RESULTS:
Microarray analysis showed 16 differentially expressed miRNAs in heart tissue from the propofol group compared with the etomidate group (10 up-regulated and 6 down-regulated), while in the blood vessels there were 25 altered miRNAs (10 up-regulated, 15 down-regulated). After verifying 12 representative miRNAs via qRT-PCR, the results showed no significant difference in the expression of
miRNAs in the heart tissue, but a significant difference in the expression of 5 miRNAs in vessel tissue between the two groups. Bioinformatics analysis predicts that the target genes of the 5 differentially expressed miRNAs are associated with chemical synapse signaling pathways.
CONCLUSIONS:
Propofol and etomidate have different effects on the expression of cardiovascular miRNAs, and further research is needed to elucidate the functional consequences of these differentially expressed miRNAs.
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