New Phytol|四川大学生科院报道CHYR1泛素化磷酸化的WRKY70以降解以平衡拟南芥的免疫力

植物控制正常生长和病原体免疫之间的平衡至关重要。然而，潜在的分子机制仍不清楚。本研究，我们报告了拟南芥中WRKY70及其伴侣CHYR1控制的这种机制。

我们发现在Pst DC3000感染WRKY70OE植物后，WRKY70靶基因SARD1的水平和WRKY70的磷酸化形式都增加。

在机制上，WRKY70在Thr22和Ser34处发生磷酸化，因此通过结合到WT盒激活SARD1表达。 当感染后恢复正常生长时，磷酸化的WRKY70通过CHYR1被26S蛋白酶体降解。

同时，非磷酸化WRKY70通过结合W（抑制活性位点）和WT（活性位点）盒抑制SARD1的表达。

WRKY70通过CHYR1控制的磷酸化介导的开关与SARD1的替代顺式元件结合有助于调节免疫和生长之间的平衡。

It is critically important for plants to control the trade‐off between normal growth and pathogen immunity. However, the underlying molecular mechanism remains largely unknown. Here we report such a mechanism controlled by WRKY70 and its partner CHYR1 in Arabidopsis.

We found that both levels of the WRKY70 target gene SARD1 and the phosphorylated forms of WRKY70 were increased in WRKY70OE plants upon Pst DC3000 infection. Mechanistically, phosphorylation of WRKY70 at Thr22 and Ser34 occurs, which therefore activates SARD1 expression through binding to a WT box.

Phosphorylated WRKY70 is degraded by 26S proteasome via CHYR1 when resuming normal growth after infection. Meanwhile, non‐phosphorylated WRKY70 represses SARD1 expression by binding to both W (inhibitory activity site) and WT (active activity site) boxes.

The binding of WRKY70 to alternative cis‐elements of SARD1 through a phosphorylation‐mediated switch controlled by CHYR1 contributes to modulating the balance between immunity and growth.

DOI:

10.1111/nph.17231

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