七氟醚后处理减轻H9C2胎鼠心肌细胞缺氧-复氧损伤并通过上调microRNA-107靶向STRADA基因
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七氟醚后处理减轻H9C2胎鼠心肌细胞缺氧-复氧损伤并通过上调microRNA-107靶向STRADA基因
翻译：牛振瑛 编辑：冯玉蓉 审校：曹莹
背景：七氟醚作为一种广泛使用的吸入性全身麻醉剂，对缺氧/复氧(H/R)损伤也有心脏保护作用。本研究旨在研究microRNA-107(miR-107)对H9C2胚胎大鼠心肌细胞七氟醚后处理(SpostC)的影响，并利用生物信息学分析方法确定七氟醚对人心肌组织保护作用的分子基础。
材料和方法：STRADA基因是从基因表达综合(GEO)数据库中鉴定获得。用七氟醚培养H9C2胎鼠心肌细胞。采用实时定量聚合酶链反应(qRT-PCR)和Western blot检测H9C2细胞STRADA和miR-107的mRNA和蛋白表达。用TargetScanHuman Version 7.2识别miR-107的靶基因，预测miR-107的STRADA 3'-UTR结合位点。双荧光素酶报告实验测定相对荧光素酶活性。分别采用MTT法和流式细胞仪检测细胞增殖率和细胞凋亡率。
结果：SpostC处理的H9C2细胞H/R损伤导致miR-107表达增加，STRADA表达降低。MiR-107与STRADA 3'-UTR特异性结合。SpostC H/R损伤H9C2细胞中miR-107表达上调，促进细胞增殖，减少细胞凋亡，下调caspase-3蛋白表达。STRADA的过表达降低了miR-107模拟物对SpostC的影响。
结论：SpostC通过靶向STRADA基因和上调microRNA-107的表达来减轻H9C2胚胎大鼠心肌细胞的H/R损伤。
原始文献来源：Jiang Q, Gu S. Sevoflurane Postconditioning Reduces Hypoxia-Reoxygenation Injury in H9C2 Embryonic Rat Cardiomyocytes and Targets the STRADA Gene by Upregulating microRNA-107[J].Med. Sci. Monit. 2020 Apr 25;26 DOI：10.12659/MSM.920849
Sevoflurane Postconditioning Reduces Hypoxia-Reoxygenation Injury in H9C2 Embryonic Rat Cardiomyocytes and Targets the STRADA Gene by Upregulating microRNA-107
Background: Sevoflurane as a widely used inhalational general anesthetic that also has a cardioprotective role in hypoxia-reoxygenation (H/R) injury. This study aimed to investigate the effects of microRNA-107 (miR-107) on sevoflurane postconditioning (SpostC) in H9C2 embryonic rat cardiomyocytes and to use bioinformatics analysis to identify the molecular basis of cardioprotection from sevoflurane in human cardiac tissue.
Material/Methods: The STRADA gene was identified from the Gene Expression Omnibus (GEO) database. H9C2 embryonic rat cardiomyocytes were cultured with sevoflurane. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to measure the mRNA expression and protein expression of STRADA and miR-107 in H9C2 cells. TargetScanHuman version 7.2 was used to identify the target gene of miR-107 and to predict the STRADA 3’-UTR binding site of miR-107. The dual-luciferase reporter assay measured the relative luciferase activity. The cell proliferation rate and cell apoptosis were measured using the MTT assay and flow cytometry, respectively.
Results: H/R injury in H9C2 cells following SpostC resulted in increased expression of miR-107 and reduced expression of STRADA. Specific binding of miR-107 was identified to STRADA 3’-UTR. Upregulation of the miR-107 in SpostC H/R injured H9C2 cells promoted cell proliferation, reduced cell apoptosis, and downregulating the protein expression of caspase-3. STRADA overexpression reduced the effects of a miR-107 mimic on SpostC.
Conclusions: SpostC reduced H/R injury in H9C2 embryonic rat cardiomyocytes by targeting the STRADA gene and by up-regulating the expression of microRNA-107.
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