NF-转录因子在番茄类黄酮生物合成中起重要作用

据报道，NF-Y转录因子在植物的许多生物学过程中起着不同的作用。然而，在植物中只有少数活性的NF-Y复合物已知，并且NF-Y复合物在类黄酮生物合成中的确切功能尚未确定。

利用各种分子、遗传和生物化学方法，我们发现NF-YB8a、NF-YB8b和NF-YB8c（NF-YB亚群）可以与NF-YC的特定亚群相互作用，然后招募两种不同的NF-YAs形成NF-Y复合物，结合CHS1启动子中的CCAAT元件。

此外，抑制特定NF-YB基因的表达可提高CHS1基因座的H3K27me3水平，并显著抑制CHS1在番茄果实成熟过程中的表达，从而导致果皮无色的粉红色果实的发育。

总而言之，通过证明NF-Y转录因子在类黄酮生物合成中起重要作用，并通过对驱动粉红色番茄果实发育的调控机制提供重要的分子见解，为我们基础知识和信息提供了重大进展 对园艺有相当大的实用价值。

NF‐Y transcription factors are reported to play diverse roles in a wide range of biological processes in plants. However, only a few active NF‐Y complexes are known in plants and the precise functions of NF‐Y complexes in flavonoid biosynthesis have not been determined.

Using various molecular, genetic and biochemical approaches, we found that NF‐YB8a, NF‐YB8b and NF‐YB8c – a NF‐YB subgroup – can interact with a specific subgroup of NF‐YC and then recruit either of two distinct NF‐YAs to form NF‐Y complexes that bind the CCAAT element in the CHS1 promoter.

Furthermore, suppressing the expression of particular NF‐YB genes increased the levels of H3K27me3 at the CHS1 locus and significantly suppressed the expression of CHS1 during tomato fruit ripening, which led to the development of pink‐coloured fruit with colourless peels.

Altogether, by demonstrating that NF‐Y transcription factors play essential roles in flavonoid biosynthesis and by providing significant molecular insight into the regulatory mechanisms that drive the development of pink‐coloured tomato fruit, we provide a major advance to our fundamental knowledge and information that has considerable practical value for horticulture.

原文链接：

https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.17112?af=R

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