茶树CsFHY3 / FAR1基因家族的全基因组鉴定，鉴定及生物和非生物胁迫下的表达分析

Genome-Wide Identification and Characterization of the CsFHY3/FAR1 Gene Family and Expression Analysis under Biotic and Abiotic Stresses in Tea Plants (Camellia sinensis)

第一作者

Zhengjun Liu and Chuanjing An

第一单位

西北农林

通讯作者

Yuefang Gao

Abstract

背景+问题：The FHY3/FAR1 transcription factor family, derived from transposases, plays important roles in light signal transduction, and in the growth and development of plants. However, the homologous genes in tea plants have not been studied.

主要研究：In this study, 25 CsFHY3/FAR1 family genes were identified in the tea plant genome via a genome‐wide study, and were analyzed based on bioinformatics.

结果1：25 CsFHY3/FAR1 genes were identified in the tea plant genome through a genome-wide study, and were classified into five subgroups based on their phylogenic relationships. Their potential regulatory roles in light signal transduction and photomorphogenesis, plant growth and development, and hormone responses were verified by the existence of the corresponding cis-acting elements.

结果2：The transcriptome data showed that these genes could respond to salt stress and shading treatment. An expression analysis revealed that, in different tissues, especially in leaves, CsFHY3/FAR1s were strongly expressed, and most of these genes were positively expressed under salt stress (NaCl), and negatively expressed under low temperature (4°C) stress.

结果3：In addition, a potential interaction network demonstrated that PHYA, PHYC, PHYE, LHY, FHL, HY5, and other FRSs were directly or indirectly associated with CsFHY3/FAR1 members.

结论：These results will provide the foundation for functional studies of the CsFHY3/FAR1 family, and will contribute to the breeding of tea varieties with high light efficiency and strong stress resistance.

摘  要

源自转座酶的FHY3 / FAR1转录因子家族在光信号转导以及植物的生长发育中起重要作用。但是，尚未研究茶树中的同源基因。在这项研究中，通过全基因组研究在茶树基因组中鉴定出25个CsFHY3 / FAR1基因，并根据它们的系统发生关系将其分为五个亚组。通过相应的顺式作用元件的存在，证实了它们在光信号转导和光形态发生，植物生长和发育以及激素反应中的潜在调节作用。转录组数据显示这些基因可以响应盐胁迫和遮光处理。表达分析表明，在不同的组织中，特别是在叶子中，CsFHY3 / FAR1s强烈表达，并且大多数这些基因在盐胁迫（NaCl）下呈阳性表达，而在低温（4°C）胁迫下呈阴性表达。此外，潜在的交互网络表明PHYA，PHYC，PHYE，LHY，FHL，HY5和其他FRS与CsFHY3 / FAR1成员直接或间接相关。这些结果将为CsFHY3 / FAR1家族的功能研究提供基础，并有助于光效率高和抗逆性强的茶品种的选育。

DOI: https://doi.org/10.3390/plants10030570

Journal: Plants

Impact Factor：2.762

Published date: 17 March 2021

END

三月

18

科学技术史上的今天

1858年3月18日，德国著名热机工程师狄塞尔出生。狄塞尔是柴油发动机的发明者，他在1893～1896年间制成并改进了他的内燃机，使其理论效率达到了百分之七十，比以前的蒸汽机有了巨大的进步。

1965年3月18日，前苏联宇航员阿列克谢·列昂诺夫走出“沃斯霍多夫-2号”宇宙飞船，在飞船外空间活动约24分钟，实现了人类第一次太空行走。

Where there is a will, there is a way!