【一作解读】TPJ-中科院遗传发育所张爱民课题组与河北农业大学刘冬成团队合作,在小麦储藏蛋白表达调控...
图1 乌拉尔图小麦整个灌浆过程不同时期的籽粒。DPA: day post anthesis.
图2 共有71个转录因子和储藏蛋白基因一起聚类在分支C1。
图3当在小麦的原生质体(上图)和未成熟胚乳(下图)中瞬时过表达时,TRIUR3_22986 (b)、TRIUR3_25137(c)、TRIUR3_27104 (d)和TRIUR3_33098(e)都可以提高储藏蛋白基因的启动子活性。本实验将单个转录因子的过表达载体和包含储藏蛋白基因启动子连接GFP的报告载体共转化小麦的原生质体和未成熟胚乳。图(a)转化的是空载体。
图4 EMSA实验表明TuNAC77与储藏蛋白基因的启动子结合(a)。双荧光素酶报告基因检测分析显示TuNAC77上调储藏蛋白基因启动子的活性(b)。
图5 和野生型相比,TaNAC77的RNA干扰株系籽粒中的储藏蛋白含量显著降低了(a和b),种子宽度也减小了(c)。
参考文献
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